ASSOCIATION OF GENETIC MARKERS IN HYPERTENSIVE DISEASE IN SIBERIAN POPULATION

Aim. To check whether the mononucleotide polymorphisms (MNP), identified in recent studies do match a risk marker criteria for arterial hypertension (AH) in Siberian population.
Material and methods. The group of subjects with AH and control group (relation 2:1) were formed from the population selection of 45-69 years old citizens of Novosibirsk (9400 subjects), which had been collected during the work in HAPIEE project. Totally 514 subjects included, of those controls – 168 (127 men and 41 women) with BP not higher than “normal” by the data of 2 and more examinations during the last years, with interval not more than 6 months. Group with AH consists of 346 subjects (206 men and 140 women) with the diagnosis of AH. Genomic DNA was extracted from venous blood by the method of phenol-chloroform extraction. Gene polymorphism was tested by PCR in real time according to the protocol of equipment manufacturer (zonds TagMan, Applied Biosystems, USA) on the device ABI 7900HT. These MNP were included into the study: rs699 gene AGT, rs5068 gene NPPB, rs17367504 gene MTHFR, rs2681492 gene ATP2B1, rs4343 gene АСЕ, rs1801253 gene ADRB1, rs11240692 gene REN, rs2846679 gene KCNJ1, rs239345 gene SCNN1B, rs1799983 gene NOS3.

Results. The risk relation to develop AH in men – carriers of GG rs699 gene AGT was1,95 (95% CI 1,08-3,53; p=0,003) comparing to the carriers of two other genotypes. Carriage of AA genotype rs699, opposite, is probably protective factor for AH development in men (HR 0,47; 95% CI 0,27-0,81; p=0,007).

Conclusion. For two from ten MNP studied the association with AH was confirmed in men: rs699 gene AGT and rs5068 gene NPPB.

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