Association of RS1800470 polymorphic variants of the transforming growth factor β1 (TGF-β1) gene with the severity of coronary atherosclerosis

Aim. To study the relationship of rs1800470 polymorphic variants of the transforming growth factor p1 (TGF-β1) gene with the severity of coronary arteries (CA) atherosclerosis.

Material and methods. The study included 256 patients with myocardial infarction (MI) (216 males and 40 females) of a Caucasoid race aged <65 years (52,1 ±8,4). Phenol-chloroform extraction was used for separating DNA from venous blood. The rs1800470 polymorphism of TGF-β1gene was tested using real-time polymerase chain reaction (PCR) (TaqMan probes, AB 7900HT). The assessment of coronary bed lesion severity was made according to the protocol of standard polyprojection coronary angiography with using of Gensini scores.

Results. For the first time in the Siberian population, the relationship of the A rs1800470 allele of the TGF-β1gene and severity of coronary atherosclerosis has been proved in men. The carriers of the rs1800470 allele A of the TGF-β1 gene had a odds ratio (OR) of the multivessel CA lesion (OR =2,84 (95% CI 1,37-5,87), p=0,004) and proximal type coronary atherosclerosis (OR =2,66 (95% CI 1,29-5,47), p=0,007). In general, the Gensini score was significantly higher in carriers of the risk A rs1800470 allele of the TGF-β1 gene: AA genotype — 57,33±41,89; AG genotype — 52,86±40,74; GG genotype — 43±28,83 (p>0,05), however, the differences were statistically significant in the upper quartile (p=0,028). OR of severe damage to CA (Gensini score >80 points) in carriers of the A rs1800470 allele of the TGF-β1 gene was 3,64 (95% CI 1,06-12,49). In women, no statistically significant correlation of the rs1800470 genotype of the TGF-β1 gene with the severity of CA lesions was identified.

Conclusion. A rs1800470 allele of TGF-β1 gene is associated with the severity of coronary atherosclerosis in men.

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