PHYSICO-CHEMICAL PROPERTIES OF RAT PLASMA LIPOPROTEINS: INFLUENCES OF SYMPATHETIC AND PARASYMPATHETIC LIVER DENERVATION, PARTIAL HEPATECTOMY AND INTRAPORTAL INTRODUCTION OF ATROPIN

Abstract

Physical-chemical properties of very low density (VLDL) and two forms of high density lipoproteins (LP) - apo E-rich HDL and apo A-rich HDL in rat blood plasma after sympathetic and parasympathetic denervation of the liver, partial hepatectomy and intraportal atropine administration were investigated. Surface potential of VLDL and E-HDL in all experimental groups was significantly lower than in sham operated controls, but the most pronounced decrease was after intraportal atropine administration and in 7 days after partial hepatectomy. In A-HDL this parameter was essentially the same. VLDL and E-HDL particle radius significantly decreased after 70% hepatectomy and intraportal atropine administration, was slightly lower in 7 days after section of splanchnic nerves and vagotomy and was invariable in the case of A-HDL. VLDL and A-HDL lipid microviscosity increased after sympathetic denervation, partial hepatectomy and vagotomy. The degree of increase for VLDL under such conditions was higher than for A-HDL. As to E-HDL this parameter did not change. Percent of protein in water equipment in VLDL and E-HDL was greater after vagotomy and atropine administration and did not change in case of bilateral section of splanchnic nerves and hepatectomy. In A-HDL, this parameter was constant.

As all these parameters reflect the LP assembling, structure and transformation, our results confirm the suggestion that autonomic neuronal factors influence the LP production, acting on post-transcriptional processes.