Analysis of VDR, ADRB1, SP4, MMP3, and MMP9 gene polymorphisms in patients with Wolff-Parkinson-White syndrome

Aim. To analyze the association of VDR (rs1544410), ADRB1 (rs1801252), SP4 (rs1011168), MMP3 (rs35068180), and MMP9 (rs11697325) gene polymorphisms with Wolff-Parkinson-White syndrome (WPW).

Material and methods. A total of 169 patients with WPW syndrome and 158 controls were included in the study. DNA was isolated from whole blood leukocytes using the phenol-chloroform extraction. Commercial kits from OOO NPF Litekh (Moscow) were used for genotyping the rs1544410 and rs11697325 polymorphisms in the VDR and MMP9 genes. Analysis of rs1011168, rs1801252, and rs35068180 was performed using the "PCR-Komplekt" amplification reagent kit ("Sintol", Moscow).

Results. When comparing the frequency of the studied polymorphisms between patients with WPW syndrome and the control group, significant differences were found for MMP3 rs35068180 and MMP9 rs11697325. No differences were found for the VDR (rs1544410), ADRB1 (rs1801252), and SP4 (rs1011168) polymorphisms. When comparing the genotype frequencies for the five studied polymorphisms between groups of patients with different clinical variants of WPW syndrome, significant differences were found for SP4 rs1011168 and MMP3 rs35068180.

Conclusion. The 5A/5A genotype of the MMP3 gene was shown to reduce the probability of WPW syndrome by almost twofold, while the AA genotype of the MMP9 gene also increased the risk of cardiovascular events by twofold.

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