Polymorphisms of genes related to inflammatory response, endothelial dysfunction, lipid metabolism, and oxidative stress in patients with coronary artery disease

Aim. To evaluate the involvement of single-nucleotide polymorphisms of genes related to the inflammatory response, endothelial dysfunction, lipid metabolism, and oxidative stress in the development of coronary artery disease (CAD).

Material and methods. The study included 560 patients, including 260 with a confirmed diagnosis of stable CAD and 300 healthy donors. DNA was isolated from peripheral blood according to a standard protocol. Genotyping of 42 polymorphic variants was performed using real-time polymerase chain reaction. Serum levels of the studied proteins were determined by enzyme-linked immunosorbent assay using commercial kits.

Results. The development of CAD is associated with CRP rs3093077, NOS3 rs1799983, EDN rs5370, CRP rs1205, LEPR rs1137100, and IL1B rs16944 polymorphic variants. Two haplotypes, rs16944A-rs1205T-rs3093077C-rs5370Grs1799983G-rs1137100A (p=0,04) and rs16944A-rs1205T-rs3093077C-rs5370G-rs1799983T-rs1137100A (p=0,032), were found to be significantly associated with a predisposition to CAD. Carriers of the A allele of CRP rs3093077 variant were characterized by higher serum C-reactive protein concentrations.

Conclusion. Thus, this study demonstrates a significant contribution of polymorphic variants of inflammatory response (IL1B rs16944, CRP rs1205, and CRP rs3093077), endothelial dysfunction (EDN rs5370 and NOS3 rs1799983), and lipid metabolism (LEPR rs1137100) genes to CAD predisposition.

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