FUNCTIONAL STATE OF MYOCARDIUM AFTER APPLICATION OF A NON-CELLULAR ALLOGENIC MATERIAL FOR STIMULATION OF REGENERATION CAPACITY IN EXPERIMENTAL INFARCTION

Aim. Evaluation of influence of the stimulated regeneration abilities of myocardium with non-cellular allogenic material (NAM) on contractility restoration in experimental post-infarction remodelling.

Material and methods. The experiment was done on the Wistar rodents with postinfarction cardiosclerosis (PICS), and PICS rodents post injection of the NAM at the moment of coronary artery stenosing. PICS was being formed during 45 days after coronary occlusion. The assessments were the formation and size of postinfarction scar of the left ventricle in rodents of different groups. Myocardial contractility was assessed by inotropic reaction of isolated papillary muscles on rest periods (4-60 s) with stimulation frequency 0,5 Hz, under conditions of perfusion with oxygnated Krebs-Henseleit solution.

Results. PICS in the rodents after injection of NAM developed only in 56%, and in comparison group 100%. The size of the scar area was 22% smaller in NAM group (p<0,05). Development of an aneurysm was 45% less in NAM group than in PICS group (p<0,05). Potentiation phenomenon for inotropic response on the periods of papillary muscles rest in PICS rodents myocardium was not observed. In increase of the duration of resting period there was increase of potentiation of inotropic response in rodents myocardium, and in this group the amplitude of response was significantly higher than changes in the other group of PICS animals.

Conclusion. Results of the study show possibility of preservation of myocardial functional capacity after stimulation of its regeneration abilities with allogenic noncellular biomaterial, composed with the technology Alloplant® in experimental infarction.

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