russjcardiolРоссийский кардиологический журналRussian Journal of Cardiology1560-40712618-7620«SILICEA-POLIGRAF» LLC10.15829/1560-4071-2014-1-ENG-45-48russjcardiol-592Research ArticleВ ПОМОЩЬ ПРАКТИЧЕСКОМУ ВРАЧУSUPPORTING A PRACTITIONERОЦЕНКА ЭФФЕКТИВНОСТИ КРИО ХРАНЕНИЯ ДОНОРСКОГО СЕРДЦА С ПОМОЩЬЮ IMAGEJ НА ОСНОВЕ АНАЛИЗА ИЗОБРАЖЕНИЙEFFICACY ASSESSMENT OF CRYO STORAGE OF DONOR HEARTS BY IMAGEJ BASED IMAGE ANALYSISLinBao-quanLinBao-quanYangSheng-shengYangSheng-shengZengZhi-yongZengZhi-yong

Division of Cardiothoracic Surgery, Fuzhou General Hospital of PLA, No.156, Xierhuan Beilu, Gulou District, Fuzhou 350025, China, Tel: +86 591 83793137, Fax: +86 591 22859388

zhiyongzeng@126.comZhuangCong-wenZhuangCong-wenDivision of Cardiothoracic Surgery, Fuzhou General Hospital of PLA, Fuzhou, China20142801201401-ENG4548Copyright © Lin B., Yang S., Zeng Z., Zhuang C., 20142014Lin B., Yang S., Zeng Z., Zhuang C.Lin B., Yang S., Zeng Z., Zhuang C.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://russjcardiol.elpub.ru/jour/article/view/592Цель

Цель. Повреждение донорского органа во время холодного хранения до трансплантации негативно сказывается на приживаемости. В данном исследовании изучаются имеющиеся доказательства эффективности различных растворов для холодного хранения, которые используются для сохранения донорских сердец in vitro до ортотопической трансплантации.

Материал и методы

Материал и методы. Систематический поиск полноценных статей, опубликованных с 1980 по август 2012, был выполнен в PubMed и Google Scholar. Детальный поиск также был сделан в любых доступных хранилищах гистопатологических образцов эндомиокардиальных биоптатов сердец.

Результаты

Результаты. Ни одно контролируемое исследование еще не было опубликовано по этой теме. Тем не менее, мы оценили всю доступную литературу, относящиеся к этой теме, и выполнили оригинальный, простой, но инновационный анализ с использованием ImageJ, Java на основе программы анализа изображения, чтобы показать огромное значение объективной оценки эффективности растворов для хранения. Наш анализ показывает, что ImageJ может быть удобно использован для получения доказательств (или отсутствия таковых) ишемического повреждения донорского сердца во время холодного хранения.

Заключение

Заключение. Даже UNOS база не дает возможность обеспечить гистопатологическими свидетельствами кардиологические биоптаты ортотопически трансплантируемых сердец. Мы, однако, показываем необходимость проведения анализа изображения, чтобы позволить установить доказательства полезности этих растворов для хранения. Мы рекомендуем получение результатов эндомиокардиальный биопсии до ортотопической трансплантации и создание реестра H&E изображений. Это единственный шаг, который направит нас к обоснованной заботе об этих пациентах, находящихся в критическом состоянии, которые нуждаются в столь сложных хирургических вмешательствах по трансплантации сердца.

Aim

Aim. Donor organ injury during cold preservation before transplantation negatively impacts graft survival. The current study was to examine available evidences for the efficacy of different cold storage solutions that are used to preserve donor hearts in vitro prior to orthotopic transplantation.

Material and methods

Material and methods. A systematic search of full-length articles published from 1980 to August 2012 was performed in PubMed and Google Scholar. Detailed searches were also made for availability of any sourceware for histopathology images of endomyocardial biopsies of stored hearts.

Results

Results. Not even a single controlled trial has been published relating to this topic. However, we assessed all available literature pertaining to this topic, and performed original, simple yet innovative analyses using ImageJ, a Java based image analyses program, to show the tremendous power to objectively examine the efficacy of the storage solution. Our analysis suggest that ImageJ may be conveniently used to obtain evidences (or lack of it) of ischemic injury of donor hearts during cold storage.

Conclusions

Conclusions. Even the UNOS database does not provide histopathological evidences of cardiac biopsies of orthotopically transplanted hearts. We, however, make the case of the need for image analyses and making availability of images to allow establishing evidence of the usefulness of these storage solutions. We recommend obtaining endomyocardial biopsy prior to orthotopic transplantation and create a registry of H&E stained slides. This is the only step that will direct us towards evidence based care of such highly critical patients who need the equally challenging surgical intervention of cardiac transplantation.

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The authors declare that there are no conflicts of interest present.