russjcardiolРоссийский кардиологический журналRussian Journal of Cardiology1560-40712618-7620«SILICEA-POLIGRAF» LLC10.15829/1560-4071-2019-7-53-62russjcardiol-3163Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL STUDIESМорфологическое исследование телоцитов в левом предсердии у пациентов с длительно персистирующей фибрилляцией предсердийMorphological study of telocytes in the left atrium in patients with long-term persistent atrial fibrillationhttps://orcid.org/0000-0003-0735-7822МитрофановаЛ. Б.MitrofanovaL. B.

Митрофанова Любовь Борисовна  — доктор медицинских наук, главный научный сотрудник НИЛ патоморфологиии.

Санкт-Петербург

St. Petersburg

lubamitr@yandex.ruhttps://orcid.org/0000-0002-1739-2480ХазратовА. О.KhazratovA. O.

Хазратов Антон Олегович — врач-патологоанатом.

Санкт-Петербург

St. Petersburg

a_hazratov@mail.ruhttps://orcid.org/0000-0001-8494-0646ГурщенковА. В.GurshchenkovA. V.

Гурщенков Александр Викторович  — кандидат медицинских наук, ассистент кафедры хирургических болезней, сердечно-сосудистый хирург.

Санкт-Петербург

St. Petersburg

gurshchenkov_av@almazovcentre.ruhttps://orcid.org/0000-0003-4709-617XОсадчийА. М.OsadchiiA. M.

Осадчий Алексей Михайлович — сердечно-сосудистый хирург.

Санкт-Петербург

St. Petersburg

osadchij_am@almazovcentre.ruhttps://orcid.org/0000-0003-2303-1144ГоршковА. Н.GorshkovA. N.

Горшков Андрей Николаевич — кандидат биологических наук, научный сотрудник, заведующий лабораторией внутриклеточного сигналинга и транспорта

St. Petersburg

angorsh@yahoo.comhttps://orcid.org/0000-0002-5252-483XГальковскийБ. Э.GalkovskyB. E.

Гальковский Борис Эдуардович — очный аспирант, врач-патологоанатом.

Санкт-Петербург

St. Petersburg

mrc4se@gmail.comФГБУ НМИЦ им. В.А. АлмазоваРоссияAlmazov National Medical Research CenterRussian FederationФГБУ НМИЦ им. В.А. АлмазоваRussian FederationФГБУ НМИЦ им. В.А. Алмазова; ФГБУ НИИ гриппа им. А.А. СмородинцеваРоссияAlmazov National Medical Research Center; Smorodintsev Research Institute of InfluenzaRussian Federation201917082019075362Copyright © Митрофанова Л.Б., Хазратов А.О., Гурщенков А.В., Осадчий А.М., Горшков А.Н., Гальковский Б.Э., 20192019Митрофанова Л.Б., Хазратов А.О., Гурщенков А.В., Осадчий А.М., Горшков А.Н., Гальковский Б.Э.Mitrofanova L.B., Khazratov A.O., Gurshchenkov A.V., Osadchii A.M., Gorshkov A.N., Galkovsky B.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://russjcardiol.elpub.ru/jour/article/view/3163

Телоциты были обнаружены в предсердиях человека в 2005г. Фибрилляция предсердий (ФП) является самым распространенным нарушением ритма сердца, сопровождаясь воспалением и фиброзом, однако морфологический анализ телоцитов при данной патологии еще не был выполнен.

Цель

Цель. Морфологический анализ телоцитов левого предсердия у пациентов с длительно персистирующей ФП.

Материал и методы

Материал и методы. Материалом исследования послужили: стенка левого предсердия (ЛП) 10 пациентов с ФП, 10 — без ФП (аутопсийный материал) и фрагменты ЛП 2 пациентов с ФП (операционный материал). Выполнялось гистологическое, иммуногистохимическое и ультраструктурное исследование, выделение культур телоцитов и конфокальная лазерная микроскопия.

Результаты

Результаты. Было подтверждено наличие телоцитов в ЛП. Морфометрический анализ выявил, что среднее количество телоцитов в ЛП достоверно меньше в 3 раза у пациентов с ФП, чем без нее (2,7±1,1 против 8,2±3,0 при х400). Корреляционный анализ показал значимые отрицательные связи количества телоцитов с ФП, фиброзом, липоматозом и воспалением.

Заключение

Заключение. Развитие воспаления и фиброза при ФП сопровождается потерей телоцитов.

The telocytes were found in the human atria in 2005. Atrial fibrillation (AF) is the most common heart rhythm disorder, accompanied by inflammation and fibrosis, however, the morphological analysis of telocytes in this pathology has not yet been performed.

Aim

Aim. To make morphological analysis of left atrial telocytes in patients with longterm persistent AF.

Material and methods

Material and methods. The study was based on the left atrial (LA) wall of 10 patients with AF, 10 without AF (autopsy material) and LP fragments of 2 patients with AF (surgical material). A histological, immunohistochemical, and ultrastructural study, telocyte culture isolation, and confocal laser microscopy were performed.

Results

Results. The presence of telocytes in the LA was confirmed. Morphometric analysis revealed that the average number of telocytes in the LP was significantly less by 3 times in patients with AF than without it (2,7±1,1 vs 8,2±3,0 with x400). Correlation analysis revealed significant negative associations of the number of telocytes with AF, fibrosis, lipomatosis, and inflammation.

Conclusion

Conclusion. The development of inflammation and fibrosis in AF is accompanied by the loss of telocytes.

телоциты левого предсердияфибрилляция предсердийконфокальная и электронная микроскопиявыделение культурleft atrial telocytesatrial fibrillationconfocal and electron microscopyculture isolationReferencesHinescu ME, Gherghiceanu M, Mandache E et al. Interstitial Cajal-like cells (ICLC) in human atrial myocardium J. Cell. Mol. Med. 2005;9(4):972-5. doi:10.1111/j1582-4934.2006.tb00306.x.Hinescu ME, Gherghiceanu M, Mandache E et al. Interstitial Cajal-like cells (ICLC) in human atrial myocardium J. Cell. Mol. Med. 2005; l 9(4):972-975 doi:10.1111/j.1582-4934.2006.tb00306.xCretoiu D, Hummel E, Zimmermann H et al. Human cardiac telocytes: 3D imaging by FIB-SEM tomography. 2014 J. Cell. Mol. Med. 2014;18(11):2157-64. doi:10.1111/jcmm.12468.Cretoiu D, Hummel E, Zimmermann H et al. Human cardiac telocytes: 3D imaging by FIB-SEM tomography. 2014 J. Cell. Mol. Med. 2014;18(11):2157-2164 doi:10.1111/jcmm.12468Gherghiceanu M, Popescu LM. Cardiac telocytes — their junctions and functional implications. Cell Tissue Res. 2012;348:265-79. doi:10.1007/s00441-012-1333-8.Gherghiceanu M, Popescu LM. Cardiac telocytes — their junctions and functional implications. Cell Tissue Res. 2012;348:265–279 doi:10.1007/s00441-012-1333-8Gherghiceanu M, Popescu LM. Cardiomyocyte precursors and telocytes in epicardial stem cell niche. J Cell Mol Med. 2010;14:871-7. doi:10.1111/j.1582-4934.2010.01060.x.Gherghiceanu M, Popescu LM. Cardiomyocyte precursors and telocytes in epicardial stem cell niche. J Cell Mol Med. 2010;14:871–7. doi:10.1111/j.1582-4934.2010.01060.xKostin S, Popescu LM. A distinct type of cell in myocardium: interstitial Cajal-like cells (ICLCs). J. Cell. Mol. Med. 2009;13(2):296-309. doi:10.1111/j.1582-4934.2008.00668.x.Kostin S, Popescu LM. A distinct type of cell in myocardium: interstitial Cajal-like cells (ICLCs). J. Cell. Mol. Med. 2009;13(2):296-309 doi:10.1111/j.1582-4934.2008.00668.xMitrofanova LB, Orshanskaya VS, Ho SY, Platonov PG. Histological evidence of inflammatory reaction associated with fibrosis in the atrial and ventricular walls in a case-control study of patients with history of atrial fibrillation. Europace. 2016;18(suppl 4): iv156-iv162 doi: 10.1093/europace/euw361.Mitrofanova LB, Orshanskaya VS, Ho SY, Platonov PG. Histological evidence of inflammatory reaction associated with fibrosis in the atrial and ventricular walls in a case-control study of patients with history of atrial fibrillation. Europace. 2016;18(suppl 4): iv156-iv162 doi: 10.1093/europace/euw361Isakov SV, Baghdasaryan AU, Gordeev ML. Operation MAZE IV: The technique of a modified version. Cardiology and cardiovascular surgery. 2017;10(5):12-15. doi:10.17116/kardio201710512-15.Isakov SV, Baghdasaryan AU, Gordeev ML. Operation MAZE IV: The technique of a modified version. Cardiology and cardiovascular surgery. 2017; 10 (5): 12-15. doi:10.17116/kardio201710512-15Richter M., Kostin S. The failing human heart is characterized by decreased numbers of telocytes as result of apoptosis and altered extracellular matrix composition J. Cell. Mol. Med. 2015;19(11):2597-606. doi:10.1111/jcmm.12664.Richter M., Kostin S. The failing human heart is characterized by decreased numbers of telocytes as result of apoptosis and altered extracellular matrix composition J. Cell. Mol. Med. 2015;19(11):2597-2606 doi:10.1111/jcmm.12664Zhao B, Chen S, Liu J et al. Cardiac telocytes were decreased during myocardial infarction and their therapeutic effects for ischaemic heart in rat. J Cell Mol Med. 2013;17(1):123-33. doi:10.1111/j.1582-4934.2012.01655.x.Zhao B, Chen S, Liu J et al. Cardiac telocytes were decreased during myocardial infarction and their therapeutic effects for ischaemic heart in rat. J Cell Mol Med. 2013;17(1):123–33. doi:10.1111/j.1582-4934.2012.01655.xMandache E, Gherghiceanu M, Macarie C et al. Telocytes in human isolated atrial amyloidosis: ultrastructural remodelling. J Cel Moll Med.2010;14:2739-47. doi:10.1111/j.1582-4934.2010.01200.x.Mandache E, Gherghiceanu M, Macarie C et al. Telocytes in human isolated atrial amyloidosis: ultrastructural remodelling. J Cel Moll Med.2010;14:2739–47. doi:10.1111/j.1582-4934.2010.01200.xManetti M, Rosa I, Messerini L et al. A loss of telocytes accompanies fibrosis of multiple organs in systemic sclerosis. J Cell Mol Med. 2014;18:253-62. doi:10.1111/jcmm112228.Manetti M, Rosa I, Messerini L et al. A loss of telocytes accompanies fibrosis of multiple organs in systemic sclerosis. J Cell Mol Med. 2014;18:253–62. doi:10.1111/jcmm.12228.Hinescu ME, Gherghiceanu M, Mandache E et al. Interstitial Cajal-like cells (ICLC) in atrial myocardium: ultrastructural and immunohistochemical characterization. J Cell Mol Med. 2006;10(1):243-57 doi:10.1111/j.1582-4934.2006.tb00306.x.Hinescu ME, Gherghiceanu M, Mandache E et al. Interstitial Cajal-like cells (ICLC) in atrial myocardium: ultrastructural and immunohistochemical characterization. J Cell Mol Med. 2006;10(1):243-57. doi:10.1111/j.1582-4934.2006.tb00306.xLiu JJ, Shen XT, Zheng X et al. Distribution of telocytes in the rat heart. J Clin Rehabil Tiss Eng Res. 2011;15:3546-8. doi:10.1007/s00441-012-1333-8.Liu JJ, Shen XT, Zheng X etal. Distribution of telocytes in the rat heart. J Clin Rehabil Tiss Eng Res. 2011;15:3546–8. doi:10.1007/s00441-012-1333-8Kostin S. Cardiac telocytes in normal and diseased hearts. Semin Cell Dev Biol. 2016;55:22-30. doi:10.1016/j.semcdb.2016.02.023.Kostin S. Cardiac telocytes in normal and diseased hearts. Semin Cell Dev Biol. 2016;55:22-30. doi: 10.1016/j.semcdb.2016.02.023Bani D, Formigli L, Gherghiceanu M et al. Telocytes as supporting cells for myocardial tissue organization in developing and adult heart. J Cell Mol Med. 2010;14(10):2531-8. doi:10.1111/j.1582-4934.2010.01119.x.Bani D, Formigli L, Gherghiceanu M et al. Telocytes as supporting cells for myocardial tissue organization in developing and adult heart. J Cell Mol Med. 2010; 14(10):2531–8. doi: 10.1111/j.1582-4934.2010.01119.x.Mitrofanova LB, Gorshkov AN, Konovalov PV et al. Telocytes in human sino-atrial node. Morphological evidence of pacemaker cell activity and the ability to conduct an electrical impulse. Russian Journal of Cardiology. 2017;22(9):42-9. doi:10.15829/1560-4071-2017-9-42-49.Mitrofanova LB, Gorshkov AN, Konovalov PV et al. Telocytes in human sino-atrial node. Morphological evidence of pacemaker cell activity and the ability to conduct an electrical impulse. Russian Journal of Cardiology 2017, 9 (149): 42–49 http://dx.doi.org/10.15829/1560-4071-2017-9-42-49Popescu LM, Gherghiceanu M, Hinescu ME, et al. Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC). J. Cell. Mol. Med. 2006;10(2):429-58. doi:10.1111/j.1582-4934.2006.tb00410.x.Popescu LM, Gherghiceanu M, Hinescu ME et al. Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC). J. Cell. Mol. Med. 2006; 10(2):429–458.doi:10.1111/j.1582-4934.2006.tb00410.xYang J, Li Y, Xue F, et al. Exosomes derived from cardiac telocytes exert positive effects on endothelial cells. Am J Transl Res. 2017;9(12):5375-87.Sheng J, ShimW, Lu J et al. Electrophysiology of human cardiac atrial and ventricular telocytes. J. Cell. Mol. Med. 2014;18(2):355-362 doi:10.1111/jcmm.12240Banciu DD, Banciu A, Radu BM. Electrophysiological Features of Telocytes. In: Wang X., Cretoiu D. (eds) Telocytes. Advances in Experimental Medicine and Biology, vol 913. Springer, Singapore, 2016. doi:10.1007/978-981-10-1061-3_19.Banciu DD, Banciu A, Radu BM. Electrophysiological Features of Telocytes. In: Wang X., Cretoiu D. (eds) Telocytes. Advances in Experimental Medicine and Biology, vol 913. Springer, Singapore, 2016. doi:10.1007/978-981-10-1061-3_19.Sheng J, ShimW, Lu J, et al. Electrophysiology of human cardiac atrial and ventricular telocytes. J. Cell. Mol. Med. 2014;18(2):355-62. doi:10.1111/jcmm.12240.Sheng J, ShimW, Lu J, et al. Electrophysiology of human cardiac atrial and ventricular telocytes. J. Cell. Mol. Med. 2014;18(2):355-62. doi:10.1111/jcmm.12240.

The authors declare that there are no conflicts of interest present.